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I recently finished all of Toaru Majutsu no Index, and have started watching Toaru Kagaku no Railgun. I'm currently in the second season of Railgun S, where we get more detail about the Sisters project than the first season of Index gave us.

It is shown that Misaka hands over her DNA map to a scientist at a young age because she believe that it will be used to help cure muscular dystrophy. She looks very young here, appearing to be younger than Last Order/20001, placing her pretty firmly in grade school.

Throughout the series, it is repeatedly said that Misaka is someone who got to level 5 through her own hard work, and started out as a level 1 just like many other students in Academy city. Logically, that would mean her ascent to fame and power is a somewhat recent phenomenon, and that she isn't genetically predisposed to become a strong esper.

Thus, the question I have is why the scientists wanted to use her DNA map for the Sisters project, when she should've been decidedly average at a young age, and nothing exemplary like she is during the anime. Surely there would have been better options, like cloning Accelerator himself?

I haven't read the novels, so maybe I was hoping that someone with more in-depth knowledge of the Toaru universe could fill me in.

Kai Tang
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If I recall correctly, an explanation for this is hinted at by Mugino later on in S, although it is addressed in more detail later on in the novels. In short, Misaka was selected because of something called the Parameter List.

It turns out some people actually are more predisposed to be espers than others. Academy City maintains a database known as the Parameter List, which seems to contain predictions/projections about how much potential an esper has. (The database is kept top-secret to prevent social unrest). Using the information in this database, the city prioritized development and resources towards the children with a higher potential, because these children would yield a higher return on investment.

So the researchers took Misaka's DNA map even though she was only a Level 1 at the time because she was projected to attain a high level based on the Parameter List.

ahiijny
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    Ah that makes sense I just finished Railgun S and I vaguely remember someone saying that Misaka always had the best resources, but I don't think the Parameter List was mentioned in the anime. Thanks! – Kai Tang Jul 13 '19 at 16:39
  • So Misaka is actually gifted to become good esper? That's quite ironic given how she criticizes people who seek level-up item or talks about the time when she was a level 1 in the 1st season. – Hp93 Aug 23 '20 at 13:39
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    @Hp93 Pretty much :P – ahiijny Aug 24 '20 at 02:14